Human CCL7 ELISA Kit
Product Name:Human CCL7 ELISA Kit
Packing:96T
Catalog No.:ELA-E0089h
Gene Name:CCL7
Detect Range:15.6-1000 pg/mL
Sensitivity:1.1 pg/mL
Target Protein Name:Human C-C motif chemokine 7
Alternative Name:C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, MCP-3, NC28, Small-inducible cytokine A7, CCL7, MCP3, SCYA6, SCYA7
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human CCL7 ELISA Kit allows for the in vitro quantitative determination of Human C-C motif chemokine 7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human CCL7 ELISA Kit has been pre-coated with an CCL7 antibody specific to Human C-C motif chemokine 7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human C-C motif chemokine 7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human C-C motif chemokine 7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm