Human CD3E ELISA Kit

Product Name:Human CD3E ELISA Kit
Packing:96T

Catalog No.:ELA-E1872h

Gene Name:CD3E

Detect Range:0.625-40 ng/mL

Sensitivity:0.23ng/mL

Target Protein Name:Human T-cell surface glycoprotein CD3 epsilon chain

Alternative Name:T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3E, T3E, CD3e
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human CD3E ELISA Kit allows for the in vitro quantitative determination of Human T-cell surface glycoprotein CD3 epsilon chain concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human CD3E ELISA Kit has been pre-coated with an CD3E antibody specific to Human T-cell surface glycoprotein CD3 epsilon chain .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human T-cell surface glycoprotein CD3 epsilon chain and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human T-cell surface glycoprotein CD3 epsilon chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human T-cell surface glycoprotein CD3 epsilon chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!