Human CD5 ELISA Kit

Product Name:Human CD5 ELISA Kit
Packing:96T

Catalog No.:ELA-E1798h

Gene Name:CD5

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Human T-cell surface glycoprotein CD5

Alternative Name:T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, CD5, LEU1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human CD5 ELISA Kit allows for the in vitro quantitative determination of Human T-cell surface glycoprotein CD5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human CD5 ELISA Kit has been pre-coated with an CD5 antibody specific to Human T-cell surface glycoprotein CD5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human T-cell surface glycoprotein CD5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human T-cell surface glycoprotein CD5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human T-cell surface glycoprotein CD5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!