Human CDKN2A ELISA Kit
Product Name:Human CDKN2A ELISA Kit
Packing:96T
Catalog No.:ELA-E0794h
Gene Name:CDKN2A
Detect Range:1.56-100 ng/mL
Sensitivity:0.98ng/mL
Target Protein Name:Human Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3
Alternative Name:Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3, Cyclin-dependent kinase 4 inhibitor A, CDK4I, Multiple tumor suppressor 1, MTS-1, p16-INK4a, p16-INK4, p16INK4A, CDKN2A, CDKN2,
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human CDKN2A ELISA Kit allows for the in vitro quantitative determination of Human Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human CDKN2A ELISA Kit has been pre-coated with an CDKN2A antibody specific to Human Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm