Human Centromere protein H, CENPH ELISA KIT

Product Name:Human Centromere protein H, CENPH ELISA KIT
Packing:96T

Catalog No.:ELI-50573h

Gene Name:CENPH

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Centromere protein H(CENPH

Alternative Name:CENPH,Centromere protein H(CENPH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Centromere protein H, CENPH ELISA KIT allows for the in vitro quantitative determination of Centromere protein H(CENPH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Centromere protein H, CENPH ELISA KIT has been pre-coated with an Centromere protein H(CENPH antibody specific to Centromere protein H(CENPH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Centromere protein H(CENPH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Centromere protein H(CENPH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Centromere protein H(CENPH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!