Human Centromere protein S, APITD1 ELISA KIT
Product Name:Human Centromere protein S, APITD1 ELISA KIT
Packing:96T
Catalog No.:ELI-10164h
Gene Name:APITD1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Centromere protein S(APITD1
Alternative Name:APITD1,Centromere protein S(APITD1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Centromere protein S, APITD1 ELISA KIT allows for the in vitro quantitative determination of Centromere protein S(APITD1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Centromere protein S, APITD1 ELISA KIT has been pre-coated with an Centromere protein S(APITD1 antibody specific to Centromere protein S(APITD1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Centromere protein S(APITD1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Centromere protein S(APITD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Centromere protein S(APITD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!