Human CERS1 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  CERS1

Protein Name  Ceramide synthase 1

Alternative Name

Human CERS1 ELISA KIT ,Human LAG1 ELISA KIT ,Human EPM8 ELISA KIT ,Human LASS1 ELISA KIT ,Human UOG1 ELISA KIT ,Human ceramide synthase 1 ELISA KIT ,Human longevity assurance (LAG1 ELISA KIT ,Human  S. cerevisiae)homolog 1 ELISA KIT ,Human longevity assurance gene 1 protein homolog 1 ELISA KIT ,Human protein UOG-1 ELISA KIT ,Human upstream of GDF1 ELISA KIT

Intended use

Human CERS1 ELISA KIT allows for the in vitro quantitative determination of CERS1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Test principle      

The microtiter plate provided in Human CERS1 ELISA KIT has been pre-coated with an CERS1 antibody specific to CERS1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CERS1 and then avidin conjugated to Horseradish Peroxidase (HRP)is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CERS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CERS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection and storage 

Serum - Use a serum separator tube (SST)and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20