Human CHIA ELISA Kit

Product Name:Human CHIA ELISA Kit
Packing:96T

Catalog No.:ELA-E9061h

Gene Name:CHIA

Detect Range:0.156-10 ng/mL

Sensitivity:0.076ng/mL

Target Protein Name:Human Acidic mammalian chitinase

Alternative Name:Acidic mammalian chitinase, AMCase, Lung-specific protein TSA1902, CHIA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human CHIA ELISA Kit allows for the in vitro quantitative determination of Human Acidic mammalian chitinase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human CHIA ELISA Kit has been pre-coated with an CHIA antibody specific to Human Acidic mammalian chitinase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Acidic mammalian chitinase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Acidic mammalian chitinase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Acidic mammalian chitinase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!