Human Chitinase- 3- like protein 2, CHI3L2 ELISA KIT

Product Name:Human Chitinase- 3- like protein 2, CHI3L2 ELISA KIT
Packing:96T

Catalog No.:ELI-10145h

Gene Name:CHI3L2

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Chitinase-3-like protein 2(CHI3L2

Alternative Name:CHI3L2,Chitinase-3-like protein 2(CHI3L2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Chitinase- 3- like protein 2, CHI3L2 ELISA KIT allows for the in vitro quantitative determination of Chitinase-3-like protein 2(CHI3L2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Chitinase- 3- like protein 2, CHI3L2 ELISA KIT has been pre-coated with an Chitinase-3-like protein 2(CHI3L2 antibody specific to Chitinase-3-like protein 2(CHI3L2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Chitinase-3-like protein 2(CHI3L2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Chitinase-3-like protein 2(CHI3L2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Chitinase-3-like protein 2(CHI3L2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!