Human CLC ELISA Kit
Product Name:Human CLC ELISA Kit
Packing:96T
Catalog No.:ELA-E1171h
Gene Name:CLC
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Human Eosinophil lysophospholipase
Alternative Name:Eosinophil lysophospholipase, Charcot-Leyden crystal protein, CLC, Galectin-10, Gal-10, Lysolecithin acylhydrolase
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human CLC ELISA Kit allows for the in vitro quantitative determination of Human Eosinophil lysophospholipase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human CLC ELISA Kit has been pre-coated with an CLC antibody specific to Human Eosinophil lysophospholipase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Eosinophil lysophospholipase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Eosinophil lysophospholipase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm