Cleaved BID/TBID ELISA KIT|Human
Catalog EF007295
Packing 48T/96T
Reactivity Human
Detect Range 7.8-500pg/ml
Cleaved BID/TBID ELISA KIT Sensitivity< 4.69pg/ml
Application Cleaved BID/TBID ELISA KIT|Human is for detecting the concentration of Human Cleaved BID/TBID in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of Cleaved BID/TBID ELISA KIT|Human
The microtiter plate provided in Cleaved BID/TBID ELISA KIT|Human has been pre-coated with an Human Cleaved BID/TBID antibody specific to Human Cleaved BID/TBID. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human Cleaved BID/TBIDantibody preparation specific for Human Cleaved BID/TBID then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Cleaved BID/TBID, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human Cleaved BID/TBIDin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that Cleaved BID/TBID ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!