Human Collagen alpha- 1(XXVI) chain, EMID2 ELISA KIT

Product Name:Human Collagen alpha- 1(XXVI) chain, EMID2 ELISA KIT
Packing:96T                                                                                  

Catalog No.:ELI-47302h                                                                        Manual

Gene Name:EMID2

Detect Range:0.78-50 ng/mL

Sensitivity:0.39ng/mL

Target Protein Name:Human Collagen alpha- 1(XXVI) chain

Alternative Name:EMID2,Human Collagen alpha- 1(XXVI) chain
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Collagen alpha- 1(XXVI) chain, EMID2 ELISA KIT allows for the in vitro quantitative determination ofHuman Collagen alpha- 1(XXVI) chain concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Collagen alpha- 1(XXVI) chain, EMID2 ELISA KIT has been pre-coated with an EMID2 antibody specific to Human Collagen alpha- 1(XXVI) chain .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Collagen alpha- 1(XXVI) chain and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Collagen alpha- 1(XXVI) chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human Collagen alpha- 1(XXVI) chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!