Human Collagen Type I Alpha 1, COL1a1 ELISA kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

COL1A1,Collagen alpha-1(I) chain,Alpha-1 type I collagen, type I collagen,EDSC; OI1; OI2; OI3; OI4; alpha-1 type I collagen; alpha1(I) procollagen; collagen alpha 1 chain type I; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone alpha-1 chain; pro-alpha-1 collagen type 1; type I proalpha 1; type I procollagen alpha 1 chain; collagen type I alpha 1

Search name

Human COL1A1 ELISA KIT ,Human Collagen alpha-1(I) chain ELISA KIT ,Human Alpha-1 type I collagen ELISA KIT ,Human  type I collagen ELISA KIT ,Human EDSC ELISA KIT ,Human OI1 ELISA KIT ,Human OI2 ELISA KIT ,Human OI3 ELISA KIT ,Human OI4 ELISA KIT ,Human alpha-1 type I collagen ELISA KIT ,Human alpha1(I) procollagen ELISA KIT ,Human collagen alpha 1 chain type I ELISA KIT ,Human collagen alpha-1(I) chain preproprotein ELISA KIT ,Human collagen of skin ELISA KIT ,Human  tendon and bone alpha-1 chain ELISA KIT ,Human pro-alpha-1 collagen type 1 ELISA KIT ,Human type I proalpha 1 ELISA KIT ,Human type I procollagen alpha 1 chain ELISA KIT ,Human collagen type I alpha 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Collagen alpha-1(I) chain concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen alpha-1(I) chain. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Collagen alpha-1(I) chain and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Collagen alpha-1(I) chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Collagen alpha-1(I) chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.