Human Complement factor B, CFB ELISA KIT

Product Name:Human Complement factor B, CFB ELISA KIT
Packing:96T

Catalog No.:ELI-06463h

Gene Name:CFB

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Complement factor B(CFB

Alternative Name:CFB,Complement factor B(CFB
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Complement factor B, CFB ELISA KIT allows for the in vitro quantitative determination of Complement factor B(CFB concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Complement factor B, CFB ELISA KIT has been pre-coated with an Complement factor B(CFB antibody specific to Complement factor B(CFB .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Complement factor B(CFB and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Complement factor B(CFB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Complement factor B(CFB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!