Human Complement factor H- related protein 1, CFHR1 ELISA KIT
Product Name:Human Complement factor H- related protein 1, CFHR1 ELISA KIT
Packing:96T
Catalog No.:ELI-07424h
Gene Name:CFHR1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Complement factor H-related protein 1(CFHR1
Alternative Name:CFHR1,Complement factor H-related protein 1(CFHR1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Complement factor H- related protein 1, CFHR1 ELISA KIT allows for the in vitro quantitative determination of Complement factor H-related protein 1(CFHR1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Complement factor H- related protein 1, CFHR1 ELISA KIT has been pre-coated with an Complement factor H-related protein 1(CFHR1 antibody specific to Complement factor H-related protein 1(CFHR1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Complement factor H-related protein 1(CFHR1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Complement factor H-related protein 1(CFHR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Complement factor H-related protein 1(CFHR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!