Human CP ELISA Kit
Product Name:Human CP ELISA Kit
Packing:96T
Catalog No.:ELA-E0909h
Gene Name:CP
Detect Range:31.2-2000 ng/mL
Sensitivity:15ng/mL
Target Protein Name:Human Ceruloplasmin
Alternative Name:Ceruloplasmin, Ferroxidase, CP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human CP ELISA Kit allows for the in vitro quantitative determination of Human Ceruloplasmin concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human CP ELISA Kit has been pre-coated with an CP antibody specific to Human Ceruloplasmin .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Ceruloplasmin and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Ceruloplasmin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm