Human CRYAA ELISA Kit

Product Name:Human CRYAA ELISA Kit
Packing:96T

Catalog No.:ELA-E1455h

Gene Name:CRYAA

Detect Range:0.78-50 ng/mL

Sensitivity:0.23ng/mL

Target Protein Name:Human Alpha-crystallin A chain

Alternative Name:Alpha-crystallin A chain, Heat shock protein beta-4, HspB4, CRYAA, CRYA1, HSPB4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human CRYAA ELISA Kit allows for the in vitro quantitative determination of Human Alpha-crystallin A chain concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human CRYAA ELISA Kit has been pre-coated with an CRYAA antibody specific to Human Alpha-crystallin A chain .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Alpha-crystallin A chain and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Alpha-crystallin A chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm