Human Crystallin Alpha A,CRYaA ELISA Kit

Product Name:Human Crystallin Alpha A,CRYaA ELISA Kit
Packing:96T

Catalog No.:ELA-E9662h

Gene Name:CRYaA

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Crystallin Alpha A,CRYaA

Alternative Name:CRYaA,Crystallin Alpha A,CRYaA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Crystallin Alpha A,CRYaA ELISA Kit allows for the in vitro quantitative determination of Crystallin Alpha A,CRYaA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Crystallin Alpha A,CRYaA ELISA Kit has been pre-coated with an Crystallin Alpha A,CRYaA antibody specific to Crystallin Alpha A,CRYaA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Crystallin Alpha A,CRYaA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Crystallin Alpha A,CRYaA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Crystallin Alpha A,CRYaA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!