Human C-terminal propeptide of COL1A1 ELISA Kit

Product Name:Human C-terminal propeptide of COL1A1 ELISA Kit

Product Name:Human C-terminal propeptide of COL1A1 ELISA Kit

Gene Name:Human C-terminal propeptide of COL1A1

Detect Range:0.312-20ng/ml

Sensitivity:0.1ng/ml

Target Protein Name:Human C-terminal propeptide of COL1A1

Alternative Name:Human C-terminal propeptide of COL1A1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human C-terminal propeptide of COL1A1 ELISA Kit allows for the in vitro quantitative determination of Human C-terminal propeptide of COL1A1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human C-terminal propeptide of COL1A1 ELISA Kit has been pre-coated with an Human C-terminal propeptide of COL1A1 antibody specific to Human C-terminal propeptide of COL1A1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human C-terminal propeptide of COL1A1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human C-terminal propeptide of COL1A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human C-terminal propeptide of COL1A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!