Human Cyclin- dependent kinase 8, CDK8 ELISA KIT
Product Name:Human Cyclin- dependent kinase 8, CDK8 ELISA KIT
Packing:96T
Catalog No.:ELI-50297h
Gene Name:CDK8
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Cyclin-dependent kinase 8(CDK8
Alternative Name:CDK8,Cyclin-dependent kinase 8(CDK8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cyclin- dependent kinase 8, CDK8 ELISA KIT allows for the in vitro quantitative determination of Cyclin-dependent kinase 8(CDK8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cyclin- dependent kinase 8, CDK8 ELISA KIT has been pre-coated with an Cyclin-dependent kinase 8(CDK8 antibody specific to Cyclin-dependent kinase 8(CDK8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cyclin-dependent kinase 8(CDK8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cyclin-dependent kinase 8(CDK8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cyclin-dependent kinase 8(CDK8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!