Human CYCS ELISA Kit
Product Name:Human CYCS ELISA Kit
Packing:96T
Catalog No.:ELA-E0594h
Gene Name:CYCS
Detect Range:0.312-20 ng/mL
Sensitivity:0.12ng/mL
Target Protein Name:Human Cytochrome c
Alternative Name:Cytochrome c, CYCS, CYC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human CYCS ELISA Kit allows for the in vitro quantitative determination of Human Cytochrome c concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human CYCS ELISA Kit has been pre-coated with an CYCS antibody specific to Human Cytochrome c .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Cytochrome c and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Cytochrome c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm