Human Cytochrome c,Cyt-C ELISA Kit
Product Name: Human Cytochrome c,Cyt-C ELISA Kit
Gene Name: Cyt-C
Detect Range: 1.56-100 ng/mL
Target Protein Name:Human Cytochrome c
Alternative Name: Cyt-C,Human Cytochrome c
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cyt-C ELISA KIT allows for the in vitro quantitative determination of Human Cyt-C concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cyt-C ELISA KIT has been pre-coated with an Human Cyt-C antibody specific to Human Cyt-C .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Cyt-C and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Cyt-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human Cyt-C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!