Human Cytochrome P450 3A43, CYP3A43 ELISA KIT
Product Name:Human Cytochrome P450 3A43, CYP3A43 ELISA KIT
Packing:96T
Catalog No.:ELI-46773h
Gene Name:CYP3A43
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Cytochrome P450 3A43(CYP3A43
Alternative Name:CYP3A43,Cytochrome P450 3A43(CYP3A43
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cytochrome P450 3A43, CYP3A43 ELISA KIT allows for the in vitro quantitative determination of Cytochrome P450 3A43(CYP3A43 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cytochrome P450 3A43, CYP3A43 ELISA KIT has been pre-coated with an Cytochrome P450 3A43(CYP3A43 antibody specific to Cytochrome P450 3A43(CYP3A43 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cytochrome P450 3A43(CYP3A43 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome P450 3A43(CYP3A43, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cytochrome P450 3A43(CYP3A43 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!