Human Cytosolic 5'- nucleotidase 3, NT5C3 ELISA KIT

Product Name:Human Cytosolic 5'- nucleotidase 3, NT5C3 ELISA KIT
Packing:96T

Catalog No.:ELI-12252h

Gene Name:NT5C3

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Cytosolic 5'-nucleotidase 3(NT5C3

Alternative Name:NT5C3,Cytosolic 5'-nucleotidase 3(NT5C3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Cytosolic 5'- nucleotidase 3, NT5C3 ELISA KIT allows for the in vitro quantitative determination of Cytosolic 5'-nucleotidase 3(NT5C3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Cytosolic 5'- nucleotidase 3, NT5C3 ELISA KIT has been pre-coated with an Cytosolic 5'-nucleotidase 3(NT5C3 antibody specific to Cytosolic 5'-nucleotidase 3(NT5C3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cytosolic 5'-nucleotidase 3(NT5C3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytosolic 5'-nucleotidase 3(NT5C3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cytosolic 5'-nucleotidase 3(NT5C3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!