DEFB4A ELISA KIT|Human

Catalog  EF000556

Packing 48T/96T

Reactivity Human

Detect Range 62.5-4000pg/ml

DEFB4A ELISA KIT Sensitivity< 37.5pg/ml

Application DEFB4A ELISA KIT|Human  is for detecting the concentration of Human DEFB4A in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

Principle of DEFB4A ELISA KIT|Human

The microtiter plate provided in DEFB4A ELISA KIT|Human has been pre-coated with an Human DEFB4A antibody specific to Human DEFB4A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human DEFB4Aantibody preparation specific for Human DEFB4A then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human DEFB4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human DEFB4Ain the samples is then determined by comparing the O.D. of the samples to the standard curve.

*Please noted that DEFB4A ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!