Human delta sleep- inducing peptide ELISA Kit

Product Name:Human delta sleep- inducing peptide ELISA Kit
Packing:96T

Catalog No.:ELA-E0708h

Gene Name:DSIP

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:delta sleep-inducing peptide

Alternative Name:DSIP,delta sleep-inducing peptide
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human delta sleep- inducing peptide ELISA Kit allows for the in vitro quantitative determination of delta sleep-inducing peptide concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human delta sleep- inducing peptide ELISA Kit has been pre-coated with an delta sleep-inducing peptide antibody specific to delta sleep-inducing peptide .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for delta sleep-inducing peptide and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain delta sleep-inducing peptide, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of delta sleep-inducing peptide in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!