Human desmin ELISA Kit

Product Name:Human desmin ELISA Kit
Packing:96T

Catalog No.:ELA-E0373h

Gene Name:Des

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:desmin

Alternative Name:Des,desmin
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human desmin ELISA Kit allows for the in vitro quantitative determination of desmin concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human desmin ELISA Kit has been pre-coated with an desmin antibody specific to desmin .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for desmin and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain desmin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of desmin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!