DFF45/DFFA ELISA KIT|Human
Catalog EF009081
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
DFF45/DFFA ELISA KIT Sensitivity< 0.094ng/ml
Application DFF45/DFFA ELISA KIT|Human is for detecting the concentration of Human DFF45/DFFA in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of DFF45/DFFA ELISA KIT|Human
The microtiter plate provided in DFF45/DFFA ELISA KIT|Human has been pre-coated with an Human DFF45/DFFA antibody specific to Human DFF45/DFFA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human DFF45/DFFAantibody preparation specific for Human DFF45/DFFA then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human DFF45/DFFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human DFF45/DFFAin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that DFF45/DFFA ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!