Human Diacylglycerol kinase kappa, DGKK ELISA KIT

Product Name:Human Diacylglycerol kinase kappa, DGKK ELISA KIT
Packing:96T

Catalog No.:ELI-31852h

Gene Name:DGKK

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Diacylglycerol kinase kappa(DGKK

Alternative Name:DGKK,Diacylglycerol kinase kappa(DGKK
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Diacylglycerol kinase kappa, DGKK ELISA KIT allows for the in vitro quantitative determination of Diacylglycerol kinase kappa(DGKK concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Diacylglycerol kinase kappa, DGKK ELISA KIT has been pre-coated with an Diacylglycerol kinase kappa(DGKK antibody specific to Diacylglycerol kinase kappa(DGKK .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Diacylglycerol kinase kappa(DGKK and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Diacylglycerol kinase kappa(DGKK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Diacylglycerol kinase kappa(DGKK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!