Human DKK1 ELISA Kit
Product Name:Human DKK1 ELISA Kit
Packing:96T
Catalog No.:ELA-E0741h
Gene Name:DKK1
Detect Range:78-5000 pg/mL
Sensitivity:31pg/mL
Target Protein Name:Human Dickkopf-related protein 1
Alternative Name:Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK, DKK1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human DKK1 ELISA Kit allows for the in vitro quantitative determination of Human Dickkopf-related protein 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human DKK1 ELISA Kit has been pre-coated with an DKK1 antibody specific to Human Dickkopf-related protein 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Dickkopf-related protein 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Dickkopf-related protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm