Human DMGDH ELISA Kit

Product Name:Human DMGDH ELISA Kit
Packing:96T

Catalog No.:ELA-E12466h

Gene Name:DMGDH

Detect Range:78-5000 pg/mL

Sensitivity:39pg/ml

Target Protein Name:Human Dimethylglycine dehydrogenase, mitochondrial

Alternative Name:Dimethylglycine dehydrogenase, mitochondrial, ME2GLYDH, DMGDH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human DMGDH ELISA Kit allows for the in vitro quantitative determination of Human Dimethylglycine dehydrogenase, mitochondrial concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human DMGDH ELISA Kit has been pre-coated with an DMGDH antibody specific to Human Dimethylglycine dehydrogenase, mitochondrial .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Dimethylglycine dehydrogenase, mitochondrial and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Dimethylglycine dehydrogenase, mitochondrial, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Dimethylglycine dehydrogenase, mitochondrial in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!