Human DNA nucleotidylexotransferase, DNTT ELISA KIT

Product Name:Human DNA nucleotidylexotransferase, DNTT ELISA KIT
Packing:96T

Catalog No.:ELI-05098h

Gene Name:DNTT

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:DNA nucleotidylexotransferase(DNTT

Alternative Name:DNTT,DNA nucleotidylexotransferase(DNTT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human DNA nucleotidylexotransferase, DNTT ELISA KIT allows for the in vitro quantitative determination of DNA nucleotidylexotransferase(DNTT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human DNA nucleotidylexotransferase, DNTT ELISA KIT has been pre-coated with an DNA nucleotidylexotransferase(DNTT antibody specific to DNA nucleotidylexotransferase(DNTT .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for DNA nucleotidylexotransferase(DNTT and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DNA nucleotidylexotransferase(DNTT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of DNA nucleotidylexotransferase(DNTT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!