Human DNA replication licensing factor MCM2, MCM2 ELISA KIT
Product Name:Human DNA replication licensing factor MCM2, MCM2 ELISA KIT
Packing:96T
Catalog No.:ELI-05736h
Gene Name:MCM2
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:DNA replication licensing factor MCM2(MCM2
Alternative Name:MCM2,DNA replication licensing factor MCM2(MCM2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human DNA replication licensing factor MCM2, MCM2 ELISA KIT allows for the in vitro quantitative determination of DNA replication licensing factor MCM2(MCM2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human DNA replication licensing factor MCM2, MCM2 ELISA KIT has been pre-coated with an DNA replication licensing factor MCM2(MCM2 antibody specific to DNA replication licensing factor MCM2(MCM2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for DNA replication licensing factor MCM2(MCM2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DNA replication licensing factor MCM2(MCM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of DNA replication licensing factor MCM2(MCM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!