Human E3 ubiquitin/ISG15 ligase TRIM25, TRIM25 ELISA KIT

Product Name:Human E3 ubiquitin/ISG15 ligase TRIM25, TRIM25 ELISA KIT
Packing:96T

Catalog No.:ELI-40165h

Gene Name:TRIM25

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:E3 ubiquitin(ISG15 ligase TRIM25(TRIM25

Alternative Name:TRIM25,E3 ubiquitin(ISG15 ligase TRIM25(TRIM25
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human E3 ubiquitin/ISG15 ligase TRIM25, TRIM25 ELISA KIT allows for the in vitro quantitative determination of E3 ubiquitin(ISG15 ligase TRIM25(TRIM25 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human E3 ubiquitin/ISG15 ligase TRIM25, TRIM25 ELISA KIT has been pre-coated with an E3 ubiquitin(ISG15 ligase TRIM25(TRIM25 antibody specific to E3 ubiquitin(ISG15 ligase TRIM25(TRIM25 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for E3 ubiquitin(ISG15 ligase TRIM25(TRIM25 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain E3 ubiquitin(ISG15 ligase TRIM25(TRIM25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of E3 ubiquitin(ISG15 ligase TRIM25(TRIM25 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!