Human EFNA1 ELISA Kit
Product Name:Human EFNA1 ELISA Kit
Packing:96T
Catalog No.:ELA-E1422h
Gene Name:EFNA1
Detect Range:0.156-10 ng/mL
Sensitivity:0.098ng/mL
Target Protein Name:Human Ephrin-A1
Alternative Name:Ephrin-A1, EPH-related receptor tyrosine kinase ligand 1, LERK-1, Immediate early response protein B61, Tumor necrosis factor alpha-induced protein 4, TNF alpha-induced protein 4, EFNA1,
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human EFNA1 ELISA Kit allows for the in vitro quantitative determination of Human Ephrin-A1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human EFNA1 ELISA Kit has been pre-coated with an EFNA1 antibody specific to Human Ephrin-A1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Ephrin-A1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Ephrin-A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm