Human EPO ELISA Kit

Product Name:Human EPO ELISA Kit
Packing:96T

Catalog No.:ELA-E0028h

Gene Name:EPO

Detect Range:0.156-10 ng/mL

Sensitivity:0.1ng/mL

Target Protein Name:Human Erythropoietin

Alternative Name:Erythropoietin, EPO
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human EPO ELISA Kit allows for the in vitro quantitative determination of Human Erythropoietin concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human EPO ELISA Kit has been pre-coated with an EPO antibody specific to Human Erythropoietin .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Erythropoietin and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Erythropoietin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm