Human Estradiol 17- beta- dehydrogenase 1, HSD17B1 ELISA KIT

Product Name:Human Estradiol 17- beta- dehydrogenase 1, HSD17B1 ELISA KIT
Packing:96T

Catalog No.:ELI-09421h

Gene Name:HSD17B1

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Estradiol 17-beta-dehydrogenase 1(HSD17B1

Alternative Name:HSD17B1,Estradiol 17-beta-dehydrogenase 1(HSD17B1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Estradiol 17- beta- dehydrogenase 1, HSD17B1 ELISA KIT allows for the in vitro quantitative determination of Estradiol 17-beta-dehydrogenase 1(HSD17B1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Estradiol 17- beta- dehydrogenase 1, HSD17B1 ELISA KIT has been pre-coated with an Estradiol 17-beta-dehydrogenase 1(HSD17B1 antibody specific to Estradiol 17-beta-dehydrogenase 1(HSD17B1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Estradiol 17-beta-dehydrogenase 1(HSD17B1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Estradiol 17-beta-dehydrogenase 1(HSD17B1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Estradiol 17-beta-dehydrogenase 1(HSD17B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!