Human Estradiol 17- beta- dehydrogenase 2, HSD17B2 ELISA KIT

Product Name:Human Estradiol 17- beta- dehydrogenase 2, HSD17B2 ELISA KIT
Packing:96T

Catalog No.:ELI-46622h

Gene Name:HSD17B2

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Estradiol 17-beta-dehydrogenase 2(HSD17B2

Alternative Name:HSD17B2,Estradiol 17-beta-dehydrogenase 2(HSD17B2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Estradiol 17- beta- dehydrogenase 2, HSD17B2 ELISA KIT allows for the in vitro quantitative determination of Estradiol 17-beta-dehydrogenase 2(HSD17B2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Estradiol 17- beta- dehydrogenase 2, HSD17B2 ELISA KIT has been pre-coated with an Estradiol 17-beta-dehydrogenase 2(HSD17B2 antibody specific to Estradiol 17-beta-dehydrogenase 2(HSD17B2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Estradiol 17-beta-dehydrogenase 2(HSD17B2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Estradiol 17-beta-dehydrogenase 2(HSD17B2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Estradiol 17-beta-dehydrogenase 2(HSD17B2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!