Human Estradiol Benzoate ELISA Kit

Product Name:Human Estradiol Benzoate ELISA Kit
Packing:96T

Catalog No.:ELA-E1262h

Gene Name:Estradiol Benzoate

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Estradiol Benzoate

Alternative Name:Estradiol Benzoate,Estradiol Benzoate
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Estradiol Benzoate ELISA Kit allows for the in vitro quantitative determination of Estradiol Benzoate concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Estradiol Benzoate ELISA Kit has been pre-coated with an Estradiol Benzoate antibody specific to Estradiol Benzoate .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Estradiol Benzoate and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Estradiol Benzoate, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Estradiol Benzoate in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!