Human EVI5- like protein, EVI5L ELISA KIT
Product Name:Human EVI5- like protein, EVI5L ELISA KIT
Packing:96T
Catalog No.:ELI-47262h
Gene Name:EVI5L
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:EVI5-like protein(EVI5L
Alternative Name:EVI5L,EVI5-like protein(EVI5L
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human EVI5- like protein, EVI5L ELISA KIT allows for the in vitro quantitative determination of EVI5-like protein(EVI5L concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human EVI5- like protein, EVI5L ELISA KIT has been pre-coated with an EVI5-like protein(EVI5L antibody specific to EVI5-like protein(EVI5L .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for EVI5-like protein(EVI5L and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain EVI5-like protein(EVI5L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of EVI5-like protein(EVI5L in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!