Human Exosome complex exonuclease RRP44, DIS3 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

DIS3,DIS3 exosome endoribonuclease and 3'-5' exoribonuclease; mitotic control protein dis3 homolog; DIS3 mitotic control homolog; 2810028N01Rik; EXOSC11; KIAA1008; RRP44; dis3p; exosome complex exonuclease RRP44; exosome component 11; ribosomal RNA-processing protein 44

Search name

Human DIS3 ELISA KIT ,Human DIS3 exosome endoribonuclease and 3'-5' exoribonuclease ELISA KIT ,Human mitotic control protein dis3 homolog ELISA KIT ,Human DIS3 mitotic control homolog ELISA KIT ,Human 2810028N01Rik ELISA KIT ,Human EXOSC11 ELISA KIT ,Human KIAA1008 ELISA KIT ,Human RRP44 ELISA KIT ,Human dis3p ELISA KIT ,Human exosome complex exonuclease RRP44 ELISA KIT ,Human exosome component 11 ELISA KIT ,Human ribosomal RNA-processing protein 44 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Exosome complex exonuclease RRP44, DIS3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to DIS3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DIS3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DIS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DIS3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.