Human Eyes absent homolog 2, EYA2 ELISA KIT
Product Name:Human Eyes absent homolog 2, EYA2 ELISA KIT
Packing:96T
Catalog No.:ELI-47555h
Gene Name:EYA2
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Eyes absent homolog 2(EYA2
Alternative Name:EYA2,Eyes absent homolog 2(EYA2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Eyes absent homolog 2, EYA2 ELISA KIT allows for the in vitro quantitative determination of Eyes absent homolog 2(EYA2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Eyes absent homolog 2, EYA2 ELISA KIT has been pre-coated with an Eyes absent homolog 2(EYA2 antibody specific to Eyes absent homolog 2(EYA2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Eyes absent homolog 2(EYA2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Eyes absent homolog 2(EYA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Eyes absent homolog 2(EYA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!