Human F12 ELISA Kit

Product Name:Human F12 ELISA Kit
Packing:96T

Catalog No.:ELA-E0694h

Gene Name:F12

Detect Range:78-5000 pg/mL

Sensitivity:34pg/mL

Target Protein Name:Human Coagulation factor XII

Alternative Name:Coagulation factor XII, Hageman factor, HAF, F12
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human F12 ELISA Kit allows for the in vitro quantitative determination of Human Coagulation factor XII concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human F12 ELISA Kit has been pre-coated with an F12 antibody specific to Human Coagulation factor XII .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Coagulation factor XII and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Coagulation factor XII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm