Human F13A1 ELISA Kit
Product Name:Human F13A1 ELISA Kit
Packing:96T
Catalog No.:ELA-E0676h
Gene Name:F13A1
Detect Range:0.312-20 ng/mL
Sensitivity:0.1ng/mL
Target Protein Name:Human Coagulation factor XIII A chain
Alternative Name:Coagulation factor XIII A chain, Coagulation factor XIIIa, Protein-glutamine gamma-glutamyltransferase A chain, Transglutaminase A chain, F13A1, F13A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human F13A1 ELISA Kit allows for the in vitro quantitative determination of Human Coagulation factor XIII A chain concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human F13A1 ELISA Kit has been pre-coated with an F13A1 antibody specific to Human Coagulation factor XIII A chain .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Coagulation factor XIII A chain and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Coagulation factor XIII A chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm