Human F8 ELISA Kit

Product Name:Human F8 ELISA Kit
Packing:96T

Catalog No.:ELA-E0841h

Gene Name:F8

Detect Range:0.312-20 ng/mL

Sensitivity:0.1ng/mL

Target Protein Name:Human Coagulation factor VIII

Alternative Name:Coagulation factor VIII, Antihemophilic factor, AHF, Procoagulant component, F8, F8C
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human F8 ELISA Kit allows for the in vitro quantitative determination of Human Coagulation factor VIII concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human F8 ELISA Kit has been pre-coated with an F8 antibody specific to Human Coagulation factor VIII .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Coagulation factor VIII and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Coagulation factor VIII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm