Human FAM214A ELISA Kit

Product Name:Human FAM214A ELISA Kit
Packing:96T

Catalog No.:ELA-E15001h

Gene Name:FAM214A

Detect Range:0.78-50 ng/mL

Sensitivity:0.39ng/ml

Target Protein Name:Human Protein FAM214A

Alternative Name:Protein FAM214A, FAM214A, KIAA1370
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human FAM214A ELISA Kit allows for the in vitro quantitative determination of Human Protein FAM214A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human FAM214A ELISA Kit has been pre-coated with an FAM214A antibody specific to Human Protein FAM214A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Protein FAM214A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Protein FAM214A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Protein FAM214A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!