Human Folic acid, FA ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Folic acid,Folacin,Vitamin B9,Vitamin Bc, folate, vitamin M, vitamin B9,vitamin Bc,folacin, pteroyl-L-glutamic acid,
pteroyl-L-glutamate,pteroylmonoglutamic acid, FA

Search name
Human Folic acid ELISA Kit, Human Folacin ELISA Kit, Human Vitamin B9 ELISA Kit, Human folate ELISA Kit, Human vitamin M
ELISA Kit, Human vitamin B9 ELISA Kit, Human vitamin Bc ELISA Kit, Human FA ELISA Kit, Human pteroyl-L-glutamic acid
ELISA Kit, Human pteroylmonoglutamic acid ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human folic acid,Folic acid concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Folic acid, During the reaction, Folic acid in the sample or standard competes with a fixed amount
of biotin-labeled Folic acid for sites on a pre-coated Monoclonal antibody specific to Folic acid. Excess conjugate and unbound sample or
standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Folic acid in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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