Human GABPA (GA- binding protein alpha chain) ELISA Kit (CUSTOM)

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

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Intended use

Human GABPA ELISA kit allows for the in vitro quantitative determination of Human GA- binding protein alpha chain concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Human GABPA ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GABPA, During the reaction, GABPA in the sample or standard competes with a fixed amount of biotin-labeled GABPA for sites on a pre-coated Monoclonal antibody specific to GABPA. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GABPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized product    Min.Order 5kits

Delivery time 4-6month

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