Human Galectin- 9 ELISA Kit

Product Name:Human Galectin- 9 ELISA Kit
Packing:96T

Catalog No.:ELA-E14899h

Gene Name:GLA9

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Galectin-9

Alternative Name:GLA9,Galectin-9
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Galectin- 9 ELISA Kit allows for the in vitro quantitative determination of Galectin-9 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Galectin- 9 ELISA Kit has been pre-coated with an Galectin-9 antibody specific to Galectin-9 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Galectin-9 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Galectin-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Galectin-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!