Human Gamma- glutamylcyclotransferase, GGCT ELISA KIT
Product Name:Human Gamma- glutamylcyclotransferase, GGCT ELISA KIT
Packing:96T
Catalog No.:ELI-37958h
Gene Name:GGCT
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Gamma-glutamylcyclotransferase(GGCT
Alternative Name:GGCT,Gamma-glutamylcyclotransferase(GGCT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Gamma- glutamylcyclotransferase, GGCT ELISA KIT allows for the in vitro quantitative determination of Gamma-glutamylcyclotransferase(GGCT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Gamma- glutamylcyclotransferase, GGCT ELISA KIT has been pre-coated with an Gamma-glutamylcyclotransferase(GGCT antibody specific to Gamma-glutamylcyclotransferase(GGCT .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Gamma-glutamylcyclotransferase(GGCT and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Gamma-glutamylcyclotransferase(GGCT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Gamma-glutamylcyclotransferase(GGCT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!